Accurate chromosomal DNA replication is fundamental for cellular function and genome integrity. In response to replication perturbations, DNA damage response and DNA damage tolerance pathways become activated and are crucial for detection and tolerance of lesions, which impact on replication completion and genome integrity. These processes are tightly interconnected with chromosome structure involving sister chromatid cohesion and chromatin architecture, influenced by structural maintenance of chromosomes complexes. The lab aims to understand the principles determining the choice of DNA repair pathways in the context of chromatin and how this affects chromosome structure and genome integrity.
Our research spans three major areas:
We are using budding yeast and vertebrate/mammalian cell lines, and employ genetic, molecular, biochemical and proteomic-based experimental strategies.