July 8th- 19th, 2019 | IFOM, Milan - Italy
All the cells in the human body contain almost identical genomes. Yet they differentiate to approximately 200 cell types, performing distinct functions and constituting specific organs and tissues. This is possible because the genome contains the full set of instructions governing the living organism, but each cell type selectively uses only a specific portion of them. Genome functionality is controlled primarily by epigenetics and transcriptional regulation mechanisms. In this context, the three-dimenzional (3D) organization of chromatin into the cell nucleus has gained increasing attention over the years, as an additional crucial layer to regulate genome functionality.
The completion of the human genome project, along with several technological advancements, allowed the development and widespread adoption of a plethora of genome-wide experimental techniques. In particular, methods based on high-throughput sequencing allow characterizing transcriptional and epigenetics regulation at unprecedented resolution and throughput. This unanticipated explosion in sequencing data has established genomics as one of the most "data intensive" sciences, thus pushing forward innovations also in related quantitative fields, including computer science, statistics and biophysics.
In this module we will experience first-hand computational data analyses techniques to extract information from functional genomics data obtained with high-throughput sequencing methods. We will especially focus on information concerning the 3D folding of chromatin as well as its relation to transcription and epigenetic regulation, to understand how these multiple layers control the genome functionality.